BenchMathLab calculators for molecular biology

Molecular weights of common lab chemicals

Updated November 8, 2026

Formula weights for 57 reagents used in molecular biology, cell culture and protein work. Click Calculate on any row to open the molarity calculator with that molecular weight filled in.

ChemicalFormulaMW (g/mol)Action
Salts
Sodium chlorideNaCl58.44Calculate →
Potassium chlorideKCl74.55Calculate →
Magnesium chloride, anhydrousMgCl₂95.21Calculate →
Magnesium chloride hexahydrateThe common lab form; check your bottleMgCl₂·6H₂O203.30Calculate →
Magnesium sulfate heptahydrateMgSO₄·7H₂O246.47Calculate →
Calcium chloride, anhydrousCaCl₂110.98Calculate →
Calcium chloride dihydrateCaCl₂·2H₂O147.01Calculate →
Manganese(II) chloride tetrahydrateMnCl₂·4H₂O197.91Calculate →
Lithium chlorideHygroscopicLiCl42.39Calculate →
Ammonium sulfate(NH₄)₂SO₄132.14Calculate →
Ammonium acetateCH₃COONH₄77.08Calculate →
Sodium acetate, anhydrousCH₃COONa82.03Calculate →
Sodium acetate trihydrateCH₃COONa·3H₂O136.08Calculate →
Potassium acetateCH₃COOK98.14Calculate →
Sodium bicarbonateNaHCO₃84.01Calculate →
Trisodium citrate dihydrateNa₃C₆H₅O₇·2H₂O294.10Calculate →
Sodium hydroxideNaOH40.00Calculate →
Potassium hydroxideKOH56.11Calculate →
Sodium azideToxic; used as a preservative at 0.02–0.05%NaN₃65.01Calculate →
Phosphates
Sodium phosphate dibasic, anhydrousNa₂HPO₄141.96Calculate →
Sodium phosphate dibasic dihydrateNa₂HPO₄·2H₂O177.99Calculate →
Sodium phosphate dibasic heptahydrateNa₂HPO₄·7H₂O268.07Calculate →
Sodium phosphate monobasic, anhydrousNaH₂PO₄119.98Calculate →
Sodium phosphate monobasic monohydrateNaH₂PO₄·H₂O137.99Calculate →
Sodium phosphate monobasic dihydrateNaH₂PO₄·2H₂O156.01Calculate →
Potassium phosphate monobasicKH₂PO₄136.09Calculate →
Potassium phosphate dibasicK₂HPO₄174.18Calculate →
Buffers
Tris basepKa 8.06 at 25 °CC₄H₁₁NO₃121.14Calculate →
Tris hydrochlorideC₄H₁₁NO₃·HCl157.60Calculate →
HEPESpKa 7.48C₈H₁₈N₂O₄S238.30Calculate →
MOPSpKa 7.14C₇H₁₅NO₄S209.26Calculate →
MES monohydratepKa 6.10C₆H₁₃NO₄S·H₂O213.25Calculate →
PIPESpKa 6.76; dissolves only once pH is raisedC₈H₁₈N₂O₆S₂302.37Calculate →
GlycineC₂H₅NO₂75.07Calculate →
Boric acidUsed in TBEH₃BO₃61.83Calculate →
EDTA disodium dihydrateDissolves only near pH 8Na₂EDTA·2H₂O372.24Calculate →
Detergents & reducing agents
SDS (sodium dodecyl sulfate)C₁₂H₂₅NaO₄S288.38Calculate →
DTT (dithiothreitol)Make fresh or store frozenC₄H₁₀O₂S₂154.25Calculate →
β-MercaptoethanolLiquid, about 14.3 MC₂H₆OS78.13Calculate →
PMSFProtease inhibitor; unstable in waterC₇H₇FO₂S174.19Calculate →
Sugars, denaturants & solvents
D-Glucose, anhydrousC₆H₁₂O₆180.16Calculate →
SucroseC₁₂H₂₂O₁₁342.30Calculate →
GlycerolLiquid, density 1.26 g/mLC₃H₈O₃92.09Calculate →
UreaCH₄N₂O60.06Calculate →
Guanidine hydrochlorideCH₅N₃·HCl95.53Calculate →
ImidazoleHis-tag elutionC₃H₄N₂68.08Calculate →
DMSOLiquid, density 1.10 g/mLC₂H₆OS78.13Calculate →
EthanolLiquid, density 0.789 g/mLC₂H₆O46.07Calculate →
L-GlutamineUnstable in solution at 37 °CC₅H₁₀N₂O₃146.14Calculate →
Cloning & selection
IPTGC₉H₁₈O₅S238.30Calculate →
X-galDissolve in DMF or DMSOC₁₄H₁₅BrClNO₆408.63Calculate →
Ampicillin sodium saltC₁₆H₁₈N₃NaO₄S371.39Calculate →
Kanamycin sulfateC₁₈H₃₆N₄O₁₁·H₂SO₄582.58Calculate →
ChloramphenicolDissolve in ethanolC₁₁H₁₂Cl₂N₂O₅323.13Calculate →
Tetracycline hydrochlorideLight-sensitiveC₂₂H₂₄N₂O₈·HCl480.90Calculate →
Puromycin dihydrochlorideC₂₂H₂₉N₇O₅·2HCl544.43Calculate →
Ethidium bromideMutagen; handle with careC₂₁H₂₀BrN₃394.29Calculate →

Check the hydrate on your bottle

Many salts are sold in several hydrated forms, and the water of crystallisation counts toward the weight. Magnesium chloride hexahydrate (MgCl₂·6H₂O, 203.30 g/mol) weighs more than twice as much per mole as anhydrous MgCl₂ (95.21 g/mol). Using the wrong one gives a solution at less than half or more than double the intended concentration. Always use the formula weight printed on the label of the bottle you are weighing from.

Liquids

For liquid reagents such as glycerol, DMSO and β-mercaptoethanol it is easier to measure volume. Convert using density: grams = mL × density. For example, 1 mL of glycerol weighs about 1.26 g, which is 13.7 mmol.

Antibiotics and salts with variable potency

Antibiotic working concentrations are usually given as µg/mL rather than molarity, and some antibiotics (such as G418 or kanamycin) are sold with lot-specific potency. Follow the supplier's certificate of analysis when exact concentration matters.